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. 2021 Nov 25;12:6867. doi: 10.1038/s41467-021-27018-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Scatter plot showing the Pearson correlation between the M1 and M2 signature scores. b Box plot showing the M1 signature score in each TAM subset. P values were calculated by the Wilcoxon test, two-sided comparisons. Multiple hypothesis correction using the Benjamini–Hochberg procedure. n = 15,049 cells. The center line, bounds of box, and whiskers represent mean, 25th to 75th percentile range, and minimum to maximum range in all boxplots. c Steady-state RNA velocity of TAM subsets. d PAGA graph showing the inferred developmental trajectories for TAM subsets. The edge width was correlated with the strength of connectivity between two subclusters. e Bar plot showing PAGA connectivity with CD14⁺ monocytes. f Bar plot showing the proportion of monocyte origin and tissue-resident microglia origin across each TAM subset (using data reported by Pombo et al. as a reference). Source data are provided as a Source Data file. g Scatter plot showing the scores by average expression of signature genes of tissue-resident microglia versus monocyte-derived macrophages. h Representative examples of tumor section stained by IF. The upper panel image indicates CD44⁺ TMEM119⁺ microglia in EPN tumor (the scale bar represents 30 μm). The dashed boxes highlight regions shown on the right side and the arrow depicts the CD44⁺ microglia in fluorescent images (the scale bar represents 100 μm). The bottom panel image indicates CD44⁺ TMEM119⁺ microglia in AEP tumor (the scale bar represents 30 μm). The dashed boxes highlight regions shown on the right side and the arrow depicts the CD44⁺ microglia in fluorescent images (the scale bar represents 100 μm). Images shown are representatives of three samples from three independent experiments. i Model of the developmental trajectory of monocyte/TAM lineages in spinal ependymomas. See also Supplementary Figs. 3 and 4.