Fig. 6. Bone integration of HA/MoS2-Ti6 in vivo.
a H&E staining of bone tissues around the implants under different conditions (n = 8 independent samples), scale bar = 100 μm. b Giemsa staining of bone tissues around the implants (n = 8 independent samples), scale bar = 100 μm. c In vivo antibacterial evaluation, (Left) the remaining bacteria colonies on Ti6 and HA/MoS2-Ti6 rods pulled out from the tissues after implantation for 14 days, (Right) the optic pictures of bacteria extracted from the implants of Ti6 and HA/MoS2-Ti6 after culturing for 24 h. d Antibacterial ability of HA/MoS2-Ti6 compared with Ti6 in vivo. (n = 8 independent samples). e The 2D and 3D pictures of HA/MoS2-Ti6 and Ti6 groups measured by Micro-CT. f Bone volume (BV)/tissue volume (TV) values of HA/MoS2-Ti6 and Ti6 groups. (n = 8 independent samples). g Methylene blue-acid magenta staining of the newly formed bone tissues on the bone-implant interface, scale bar = 100 μm. h Bone area ratios of samples calculated from the methylene blue-acid magenta staining. (n = 3 independent samples). i Safranin-O/Fast Green staining. The green color is osteogenesis, and the red or orange color is cartilage. The scale bar is 100 μm. j Histomorphometric measurements of osteogenesis. The area was taken from 20 μm around the implant. The control was Ti6 + S. aureus group. (n = 3 independent samples). k Biological assessment of heart, liver, spleen, lung, and kidney (n = 8). The scale bar is 100 μm. d, f, h, j Data represented as mean ± standard deviations from a representative experiment. Error bar represents the standard deviation. p-values were generated by two-way ANOVA with Dunnett’s multiple comparison test. Source data are provided as a Source Data file.