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. 2021 Nov 25;11:22920. doi: 10.1038/s41598-021-01929-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Results on test images. The c-ResUnet (no AO) correctly handles evident artifacts (a, topleft corner), while the c-ResUnet fails with more problematic structures (b). Notice how false positives (c, red boxes) look like target cells. Likewise, the objects discarded (d, blue boxes) are similar to other stains that were not annotated.