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. 2021 Nov 25;11:22911. doi: 10.1038/s41598-021-02308-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative metabolic activity of MTX and FA-treated organoids at different stages of treatment. MTX-treated organoids were supplemented with FA at different time points (0, 24, 48 and 72 h) with 5 µg/mL FA (n = 4). Metabolic activity was measured by WST-1 assay. All data are presented as Mean ± SEM. ####p < 0.0001 represents the significant differences observed between untreated organoids and MTX-treated organoids. ^^^p < 0.001 represents the significant differences between FA-treated organoids and MTX-treated organoids. **p < 0.01 and ****p < 0.0001 represent the differences observed between MTX-treated organoids and MTX/FA-treated organoids.