Fig. 5. SCoV-2 S and T403R RaTG13 S allow entry with human but not bat ACE2.

a HEK293T cells expressing indicated ACE2 variants or b Tb 1 Lu, Tadarida brasiliensis derived lung epithelial and Ri 1 Lu huACE2 Rhinolophus affinis derived lung epithelial cells expressing human ACE2 were infected with VSVΔG-GFP pseudotyped with SCoV-2, RaTG13 or indicated mutant S. Quantification by automatic counting of GFP positive cells. Bars represent the mean of three independent experiments (±SEM). a, b Two-tailed Student’s t-test with Welch’s correction.