KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Canine glioma biopsies	Oncovet Clinic	oncovet-clinical-research.com
Chemicals, peptides, and recombinant proteins
Optima LC/MS grade acetonitrile	Fisher	Cat# A955-4
Optima LC/MS grade methanol	Fisher	CAS 67-56-1
Xylene	Fisher	CAS 1330-20-7
Trifluoroacetic acid	Biosolve	CAS 76-05-1
Ammonium bicarbonate	Sigma	CAS 1066-33-7
2,5 dihydroxybenzoic acid (DHB)	Sigma	CAS 490-79-9
Dako Mounting Medium	Agilent	Cat# CS70330-2
4′,6-diamidino-2-phenylindole (DAPI)	Sigma	CAS 28718-90-3
poly-D-lysine hydrobromide	Sigma	CAS 27964-99-4
bovine serum albumin (BSA)	Sigma	CAS 9048-46-8
Biotech grade Tris buffer	Interchim	P/N UP158387
Formaldehyde	Sigma	CAS 50-00-0
HCl ampules, sequencing grade	ThermoFisher	Cat# 24308
Formic acid ampules	ThermoFisher	Cat# A11710X1-AMP
Fluoresceine-tagged Sambuccus nigra agglutinin (SNA)	Laboratory of Prof. Anne Harduin-Lepers (U Lille); Vector laboratories	Cat # FL-1301-2
Sequence grade modified trypsin	Promega	V5111
Glycerol free PNGaseF	New England Biolabs	P0705S
Ethanol, 200 proof	Fisher	CAS 64-17-5
Pierce Ultrapure Water	ThermoFisher	Cat# 51140
Deposited data
Glycoproteomic.raw files	This Study	PRIDE: PXD025537
Byonic glycoproteomic search results	This Study	PRIDE: PXD025537
Annotated glycoproteomic results	This Study	PRIDE: PXD025537
MALDI-MSI.raw files	This Study	PRIDE: PXD025826
Software and algorithms
Xcalibur software	ThermoFisher
Byonic	ProteinMetrics	https://proteinmetrics.com/
GlycoMod	ExPasy	web.expasy.org/glycomod/
SCiLS MALDI-MSI software	Bruker	https://scils.de/
Other – Instrumentation
Rapiflex MALDI-MSI	Bruker	n/a
MALDI-LTQ-Orbitrap	Thermo	n/a
Orbitrap Fusion Tribrid	Thermo	n/a
Dionex Ultimate 3000 HPLC	Thermo	n/a
Fluorescence microscope	Leica Biosystems	n/a
Centrivap vacuum concentrator	Labconco	n/a
Chemical inkjet printer (CHIP 1000)	Shimadzu	n/a