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. 2021 Nov 25;12:6839. doi: 10.1038/s41467-021-27172-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Top 5 most significant GO terms and P values (-LogP) of 18 germ cell clusters are indicated by distinct colors, respectively. b Heatmap of 97 cell-cycle-related genes in mouse male germ cells. The color key from black to yellow indicates low to high expression levels. L, leptotene; Z, zygotene; P, pachytene; D, diplotene; MI, metaphase I; RS, round spermatids. c Proportion germ cells in one of three cell-cycle phase. d Line plots showing the relative expression levels (log(TPM/10+1)) of previously known core pluripotency factors, and spermatogonial factors in each cell cluster.