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. 2021 Nov 25;4:1325. doi: 10.1038/s42003-021-02859-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Violin plot showing the expression of CCR6, CXCR3, KI67, GATA3 in CD4⁺ TC between preSU and postSU groups in CyTOF. b Violin plot showing the expression of CXCR3, CCR6 in BC between preSU and postSU groups in CyTOF. c Volcano plot showing DEGs of T-mito between preSU and postSU groups. d UpSet Plot showing the integrated comparative analysis of upregulated DEGs in BC subsets. e Representative GO biological process and pathways enriched in upregulated DEGs based on functional enrichment analysis in CD4⁺ TC, T-mito and BC subsets. f The heatmap showing the enhanced activity of TFs predicted by TRRUST analysis in CD4⁺ TC, T-mito and BC subsets. g Network visualization of the predicted transcriptional regulatory networks enhanced by SU using TRRUST tool. h Violin plot showing the expression of CDKN1A, PIM1, FOS in CD4 T_EM between preSU and postSU groups in scRNA-seq. For the box plot within each violin plot, middle lines indicate median values, boxes range from the 25th to 75th percentiles. Significance in a, b and h was calculated using two-sided Wilcoxon test as implemented in the function “compare_means” with default parameters; **P < 0.01, ****P < 0.0001.