Figure 2.

Nested RT-PCR analysis of p65 iso5 expression in RNA samples from human and mouse brain in different mouse tissues. (a) First round PCR reaction was performed with specific oligonucleotides for the exon − 1 and 3′UTR (untranslated region). For human and mouse RNA, subsequent RT-PCR reactions were performed with oligonucleotide specific for exon-1 and exon 5; exon 7; exon 9; exon 10 (the entire transcript). PCR analysis of mouse brain RNA were performed with specific primers for: exon − 1 and exon 2; exon − 1 and exon 0; exon 0 and exon 2. The + and − symbols indicate the presence and the absence respectively of reverse transcriptase in the reaction. (b) Nested RT-PCR performed on mouse RNA samples extracted from different tissues. First round PCR was performed with primers located on exon − 1 and 3′UTR region, second round PCR was performed with oligonucleotides specific for exon 2 and 10. The − symbol indicates the absence of reverse transcriptase in the reaction (representative of each sample). The full length images of the gels are available in the “Supplementary Information” file.