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. 2021 Nov 25;12:6842. doi: 10.1038/s41467-021-27235-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Spore viability of the indicated strains following heat treatment (80 °C for 20 min) and colony formation on LB agar plates. b Spore germination in response to 1 mM l-alanine as assessed by the percent reduction in OD₆₀₀. Representative data from one of three biological replicates with two technical replicates are shown. Error bars indicate ± SD of two technical replicates. c Impact of GerAB variants on the stability of GerAA and GerAC in dormant spores. Immunoblot analysis of spore lysates of the indicated strains using anti-GerAA and anti-His antibodies. SleB was used to control for loading. Molecular weight markers in Kd are indicated on the right. Representative data from one of three biological replicates are shown. Source data are provided as a Source Data file.