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. 2021 Nov 19;15(2):dmm048953. doi: 10.1242/dmm.048953

Fig. 3.

Fig. 3.

Human KRASG12V transgene expression is associated with Drosophila hemocyte immune function deficits. (A) Hemocytes (green) from control (Hml-Gal4, UAS-GFP) and KRASG12V (Hml-Gal4, UAS-GFP; UAS-KRASG12V) third-instar larvae co-incubated with fluorescent pHrodo Red-tagged S. aureus or E. coli (red). Scale bars: 10 μm. (B) Quantification of phagosome area in control and KRASG12V hemocytes co-incubated with fluorescent pHrodo Red-tagged S. aureus or E. coli (n=100; results presented as mean±s.d.; *P<0.05; unpaired Student's t-test). (C) Survival curves of control and KRASG12V adult flies infected with pathogenic P. luminescens bacteria or sterile PBS (vehicle) at 18°C. Forty flies were analyzed for each group. (D) Quantitative RT-PCR showing the relative expression level of genes involved in antibacterial defense in hemocytes from control compared to KRASG12V flies (n=3 replicates in each group; results presented as mean±s.d.; *P<0.05; unpaired Student's t-test). CecC, Cecropin C; modSP, modular serine protease; grass, Gram+-specific serine protease; LysX, Lysozyme X.