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. 2021 Nov 17;118(47):e2113757118. doi: 10.1073/pnas.2113757118

Fig. 1.

Fig. 1.

AGO1 genetically functions with FCA and FLD to repress FLC. (A) Co-IP in nuclear extracts from wild-type seedlings (Control) or seedlings expressing SDG26-TAP. IP: immunoprecipitation; IB: immunoblot. Asterisk indicates nonspecific signal. (B) Expression of spliced and unspliced FLC relative to UBC in Col-0 and ago1-25 seedlings. Data are normalized to wild-type Col-0. Data are presented as the mean ± SEM (n = 3). Asterisks indicate significant differences between the indicated plants (**P ≤ 0.007136, *P ≤ 0.031062, two-tailed t test). (C) Expression of spliced and unspliced FLC relative to UBC in various genotypes. Data are normalized to Col-0. Data are presented as the mean ± SEM (n = 3 to 5). Two-tailed P value from multiple t test corrected by Holm–Sidak method; ns, not significant. (D) Expression of spliced and unspliced FLC relative to UBC in Col FRI and ago1-25 FRI seedlings. Data are normalized to Col FRI. Data are presented as the mean ± SEM (n = 6). Asterisks indicate significant differences between the indicated plants (*P ≤ 0.029432, ****P ≤ 0.000551, two-tailed t test). (E) Expression of spliced and unspliced FLC relative to UBC in C2 and ago1-25 C2 seedlings. Data are normalized to C2. Data are presented as the mean ± SEM (n = 3). Asterisks indicate significant differences between the indicated plants (***P ≤ 0.002662, ****P ≤ 0.000588, two-tailed t test).