Fig. 5.

Model for how modulation of antisense RNA processing determines sense transcriptional output. Antagonistic assembly of cotranscriptional machinery with RNA Pol II influences transcript processing. During COOLAIR transcription, FCA, AGO1, and components of NTC/NTR link to RNA Pol II and promote proximal COOLAIR. THO/TREX dynamically competes for RNA Pol II to promote distal COOLAIR. The differential composition of the cotranscriptional regulators with the Pol II complex influences the chromatin state locally, which in turn influences the transcriptional output of the whole locus.