Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Sep;20(18):6677–6685. doi: 10.1128/mcb.20.18.6677-6685.2000

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2000, American Society for Microbiology

PMC Copyright notice

FIG. 3 — FACS analysis of B- and T-cell development in HEB^bm mice. (A) Analysis of bone marrow (top) and lymph node (bottom) cells from adult HEB^bm/+ and HEB^bm/bm mice. CD43 and B220 markers were used to separate pro-B cells (CD43⁺ B220⁺), pre- and immature B cells (CD43^low B220⁺), and mature B cells (CD43⁻ B220^high) in the bone marrow. TCRβ and B220 markers were used to separate T and B cells, respectively, in the lymph nodes. The relative percentage for each population is shown in the plots. Inguinal lymph nodes were collected from each animal, with the total cell numbers given on top of the plots. (B) E18.5 fetal thymus of wild-type, HEB^bm/+, and HEB^bm/bm thymocytes were analyzed in three separate stainings for CD4 and CD8 (top) and TCRα/β and TCRγ/δ (bottom) markers. CD4-, CD8-, and TCR-positive cells were gated out in the bottom panel. Cell counts of total thymocytes and individual populations are shown in the plots. Data are representative of multiple tests (n = 7 for HEB^bm/bm). Events displayed for all plots in A and B are after size and 7AAD gating, which eliminates nonlymphoid and dead cells, respectively. (C) Cell count of E18.5 fetal thymus collected from five litters of timed mating between HEB^bm heterozygous mice. Numbers of fetuses for each genotype included in the analysis are shown next to the genotype name in the chart. Means and standard deviations (in parentheses) for wild type, HEB^bm/+, and HEB^bm/bm are 3.3 (1.4) × 10⁶, 3.6 (1.5) × 10⁶, and 0.2 (0.2) × 10⁶, respectively. Two-tailed t test shows a statistical difference between wild type and HEB^bm/bm (P = 1.7 × 10⁻⁵) and no significant difference between wild type and HEB^bm/+ (P = 0.7).

FIG. 3 — FACS analysis of B- and T-cell development in HEB^bm mice. (A) Analysis of bone marrow (top) and lymph node (bottom) cells from adult HEB^bm/+ and HEB^bm/bm mice. CD43 and B220 markers were used to separate pro-B cells (CD43⁺ B220⁺), pre- and immature B cells (CD43^low B220⁺), and mature B cells (CD43⁻ B220^high) in the bone marrow. TCRβ and B220 markers were used to separate T and B cells, respectively, in the lymph nodes. The relative percentage for each population is shown in the plots. Inguinal lymph nodes were collected from each animal, with the total cell numbers given on top of the plots. (B) E18.5 fetal thymus of wild-type, HEB^bm/+, and HEB^bm/bm thymocytes were analyzed in three separate stainings for CD4 and CD8 (top) and TCRα/β and TCRγ/δ (bottom) markers. CD4-, CD8-, and TCR-positive cells were gated out in the bottom panel. Cell counts of total thymocytes and individual populations are shown in the plots. Data are representative of multiple tests (n = 7 for HEB^bm/bm). Events displayed for all plots in A and B are after size and 7AAD gating, which eliminates nonlymphoid and dead cells, respectively. (C) Cell count of E18.5 fetal thymus collected from five litters of timed mating between HEB^bm heterozygous mice. Numbers of fetuses for each genotype included in the analysis are shown next to the genotype name in the chart. Means and standard deviations (in parentheses) for wild type, HEB^bm/+, and HEB^bm/bm are 3.3 (1.4) × 10⁶, 3.6 (1.5) × 10⁶, and 0.2 (0.2) × 10⁶, respectively. Two-tailed t test shows a statistical difference between wild type and HEB^bm/bm (P = 1.7 × 10⁻⁵) and no significant difference between wild type and HEB^bm/+ (P = 0.7).