FIG. 5.
Adoptive transfer test of wild-type (left) and HEBbm/bm (right) stem cells into wild-type hosts. Bone marrow cells (1 to 2 × 105) from wild-type or HEBbm homozygous neonates were transferred into C57BL/6 Ly5A congenic mice irradiated with 1,100 rads. Bone marrow cells, splenocytes, and thymocytes were collected and analyzed 1 month after adoptive transfer. A four-color flow cytometry test was carried out with CD45.2-fluorescein isothiocyanate (donor-specific marker) and 7AAD included in all experiments. The CD45.2+ 7AAD− cells were analyzed with CD43-pe and B220-apc markers for bone marrow cells, Mac1-pe and B220-apc for splenocytes, and CD8-pe and CD4-apc for thymocytes. Total thymocytes recovered from the wild type transfer and HEBbm/bm mutant transfer were indicated on the top of the plots. Data are representative of three separate sets of transfer experiment.