FIG. 7.
The AND TCR transgene cannot rescue the HEBbm mutation. (A) E18.5 fetal thymuses were analyzed by costaining with CD8, CD4, and TCR antibodies. Size plots are shown with genotypes indicated on the top. Cells highlighted in the R1 gate in the size plots (top panel) are displayed for CD4/CD8 staining (bottom panel). Data are representative of multiple litters. (B) Lymph nodes of single and double transgenic mice were analyzed with CD8, CD4, and 7AAD. The percentages of CD4 single positive and CD8 single positive populations are indicated in the CD4/CD8 plots. The total cell number of inguinal lymph nodes for each genotype analyzed is given on the top. Similar results were obtained from the analysis of splenocytes.