Fig. 1.
Diabetes incidence in sublines of the NOD/Nck strain. (A) Incidence of T1D at generation 3 in five distinct sublines (A to E) established from the NOD/Nck strain by brother–sister mating of mice within individual litters. For further studies we concentrated on sublines A and B, which we named NOD/NckL (for low incidence) and NOD/NckH (for high incidence). (B) Incidence of T1D in NOD/NckH and NOD/NckL male mice followed from generation 3 up to generation 34. ****P < 0.0001, ***P < 0.001, *P < 0.05. (C) Incidence of T1D in NOD/NckH and NOD/NckL male mice derived from embryos frozen at generation 7 and revitalized. Actuarial survival curves were compared using the log-rank (Mantel–Cox) statistical test.