Fig. 4.

The NOD/Nck^H Dusp10 variant acts within islets to confer susceptibility to T1D and results in down-regulation of type I interferon signature genes. (A) Incidence of T1D in female NOD/Nck^H and NOD/Nck^L recipients after adoptive transfer of diabetogenic lymphocytes of the reciprocal genotype at 24 to 48 h after birth. Groups of experimental and control (sham injected) recipients were equally distributed within the litters. NOD/NcK^L recipients were less sensitive to T1D transfer than NOD/Nck^H recipients. (B) Comparative transcriptome analysis of purified islets of Langerhans from NOD/Nck^H and NOD/Nck^L mice at 3 wk of age. Identification of 27 down-regulated genes in NOD/Nck^H mice that included various type I interferon signature genes. (C) The differential expression of type I interferon signature genes was validated by qPCR targeting Cd274 (PD-L1) and four additional well-known type I interferon signature genes (Ifit3, Irf7, Irf9, and Oasl2). **P < 0.01, *P < 0.05.