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. 2021 Nov 18;118(47):e2107543118. doi: 10.1073/pnas.2107543118

Fig. 2.

Fig. 2.

Normal distribution of Class I COs requires AtFANCM, AtFIGL1, and AtRMI1. (A–F) Immunostaining of AtHEI10 at diakinesis in WT, Atfancm-3, Atfigl1-17, Atfigl1-18, Atrmi1-2, and Atrmi1-5. DAPI-stained chromosomes are shown in white, and AtHEI10 foci are shown in green. (G) Distributions of the number of AtHEI10 foci on homologous chromosome pairs (bivalents) in WT, Atfancm-3, Atfigl1-17, Atfigl1-18, Atrmi1-2, and Atrmi1-5, compared to (WT + Poisson)/2, and Poisson expectations. n.s., not significant, **P < 0.01 χ2 test. (H) CVs in the distributions of AtHEI10 foci per homolog pairs in WT, Atfancm-3, Atfigl1-17, Atfigl1-18, Atrmi1-2, Atrmi1-5, and the Poisson expectation. The red dash line indicates the average of the WT and Poisson CVs. (I) Meiotic chromosome morphologies of WT, Atfancm-3, Atfigl1-17, and Atfigl1-18 at metaphase I. (Scale bars, 5 μm.) (J) The distance between adjacent AtHEI10 foci in WT, Atfancm-3, Atfigl1-17, and Atrmi1-5, expressed as a percentage of bivalent length. Error bars indicate SD. **P < 0.01 two-tailed Mann–Whitney U test.