Fig. 3.

AtFANCD2, AtFANCM, AtFIGL1, and AtRMI1 regulate the distribution of Class I CO among chromosomes similarly. (A–H) Immunostaining of AtHEI10 at diakinesis in WT, Atfancd2-1, Atfancm-3 Atfigl1-17, Atfancm-3 Atrmi1-5, Atfigl1-17 Atrmi1-5, Atfancm-3 Atfancd2-1, Atfigl1-17 Atfancd2-1, and Atrmi1-5 Atfancd2-1. DAPI-stained chromosomes are shown in white, and AtHEI10 foci are shown in green. (I) Distributions of the number of AtHEI10 foci on homologous chromosome pairs (bivalents) in WT, Atfancd2-1, Atfancm-3 Atfigl1-17, Atfancm-3 Atrmi1-5, Atfigl1-17 Atrmi1-5, Atfancm-3 Atfancd2-1, Atfigl1-17 Atfancd2-1, and Atrmi1-5 Atfancd2-1, compared to (WT + Poisson)/2 and Poisson expectations. n.s., not significant, **P < 0.01 χ² test. (Scale bars, 5 μm.)