Fig. 3.
mpk12/4GC double mutants close stomata rapidly in response to VPD increase. (A and B) Time-resolved stomatal conductance (Gs) in response to increases in VPD in Col-0 (WT), mpk12/4GC-1, and mpk12/4GC-2 double-mutant leaves under high light (450 μmol·m−2·s−1 red light combined with 50 μmol·m−2·s−1 blue light). VPDs are shown on top of the time axis in each panel. Note that WT controls in A−D are the same, because mutants and WT were grown in parallel and analyzed within the same experimental sets. (C and D) Changes in stomatal conductances (ΔGs) were calculated as the difference between actual stomatal conductance and the average steady-state stomatal conductance at ∼0.8 kPa. (E and F) Average rate constants and average half-response times in response to VPD elevations are calculated and presented. Data represent mean ± SEM. Different letters represent significant differences (P < 0.05) analyzed by one-way ANOVA followed by Holm–Sidak multiple comparisons. n = 5 leaves for each genotype.