Fig. 6.
Stomatal VPD response is slower in B3-family Raf-like kinase m3kδ1/δ5/δ6/δ7 quadruple mutants. (A and B) Time-resolved stomatal conductance (Gs) in response to increases in VPD in Col-0 (WT), Raf-like m3kδ1/δ5-1/δ6-1/δ7 quadruple-mutant, and m3kδ1/δ5-2/δ6-1/δ7 quadruple-mutant leaves under high light (450 μmol·m−2·s−1 red light combined with 50 μmol·m−2·s−1 blue light). VPDs are shown on top of the time axis in each panel. (C–F) Average rate constants (C and D) and average half-response times (E and F) in response to VPD elevations were calculated and presented. Data represent mean ± SEM. n = 5 for each genotype in A. n = 3 for WT and n = 4 for the m3kδ1/δ5-2/δ6-1/δ7 quadruple mutant in B. *P < 0.05; **P < 0.01 (analyzed by Student’s t test compared to WT control). Data represent one of two independent sets of experiments in each mutant allele showing similar results.