Table 3.
MICs of tobramycin and CP762 alone and in combination
Checkerboard assays were performed in LB against 11 isolates of P. aeruginosa. MICs were determined for agent alone or in combination. Data are the mean of 6 replicates, minimal and maximal values between brackets when applicable (i.e., when MICs varied between replicates). The mean FICI were then calculated and used to plot data.
| Strains | MIC tobramycin (mg/L) |
MIC CP762 (mg/L) |
Mean FICI | |||||
|---|---|---|---|---|---|---|---|---|
| Alone | In combination | Alone | In combination | |||||
| Reference strains | PAO1 | 2 | 2 | 32 | 32 | 2 | ||
| PA14 | 2 | 2 | 32 | 32 | 2 | |||
| Clinical isolates | RP73 | 8 | 5.0 (2 – 8) | 4 | 2.7 (2 – 4) | 1.2 | ||
| CF02 | 1 | 0.75 (0.5 – 1) | 16 | 12.0 (8 - 16) | 1.5 | |||
| CF08 | 2 | 1.8 (1 – 2) | 32 | 29 (16 – 32) | 1.8 | |||
| CF10 | 1 | 1 | 32 | 32 | 2 | |||
| CF11 | 27 (16 - 32) | 24.0 (16 – 32) | 6.7 (4 – 8) | 6.0 (4 – 8) | 1.8 | |||
| CF13 | 2 | 2 | 32 | 32 | 2 | |||
| CF15 | 2 | 2 | 32 | 32 | 2 | |||
| CF19 | 32 | 27 (16 – 32) | 6.7 (4 – 8) | 5.3 (4 – 8) | 1.7 | |||
| CF26 | 2.7 (2 - 4) | 3.7 (2 – 4) | 32 | 32 | 2.5 | |||