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Ectopic overexpression of WUS or ARF5Δ induces DR5:GFP in root tips. One-week-old seedlings were transferred to plates with varying amounts of β-estradiol and imaged in brightfield and GFP fluorescence after 24 h. Wild-type (Col-0) plants were transformed with XVE:GFP (left panel). Plants carrying DR5:GFP were transformed with XVE:ARF5Δ and XVE:WUS (center and right panels, respectively). The scale bar represents 150 µm.
