Figure 1.

Antihistamines potentiate dexamethasone-induced GR transrepression of a gene-reporter system. (A) HEK-293T cells were co-transfected with IL6-Luc, GR and H1R coding constructs and were incubated with 2000 UI/mL TNF-α for 4 h and exposed to 10 μM antihistamines for 10 min and to 1 nM dexamethasone (DEX) for 24 h (filled black bars) or not (empty white bars), as indicated. (B) Data express inhibition of the TNF-α-induced response as fold of the DEX effect. Luciferase activity was determined as described in the methodology section. Results are mean +/− SD of five independent experiments performed in triplicates. Statistical significance was denoted using letters above bars. Means indicated with a common letter are not significantly different. Likewise, means not sharing any letter are significantly different.