Table 2.

The affinities between the constructs and HER2 or SAs.

Affibody Carrier	HER2 (nM)	HSA (nM)	MSA (nM)
Z	0.45 ± 0.04 a	N.D. b	N.D.
Z-ABD	0.41 ± 0.01	0.14 ± 0.03	3.1 ± 0.2
ABD-Z	2.3 ± 0.1	0.95 ± 0.03	6.2 ± 1.2
Z-Z	0.031 ± 0.007	N.D.	N.D.
Z-ABD-Z	0.71 ± 0.02	0.62 ± 0.27	3.6 ± 0.3
ABD-Z-Z	0.99 ± 0.07	0.62 ± 0.02	3.3 ± 0.2
Z-Z-ABD	0.84 ± 0.02	0.62 ± 0.03	3.6 ± 0.2

^a Each value is the average of three independent measurements ± 1 SD. Representative sensorgrams used for the measurements are shown in Supplementary Figure S1. ^b Not determined.