Table 2.

Effects of IFN-γ stimulation on PD-L1 in PC-cell lines.

Cell Lines	Origin	IFN-γ Dose	Treatment Time	Detection Method	Effect on PD-L1
BPH1 [13]	Human	50 mg/mL	24 h	WB	=
CWR22Rv1 [94]	Human	100 U/mL	48 h	FC	↑
CWRR-1 [13]	Human	50 mg/mL	24 h	WB	=
C4-2 [13]	Human	50 mg/mL	24 h	WB	=
DU145 [126]	Human	0.5–10–20 ng/mL	48 h	FC	=
DU145 [10]	Human	Not reported	Not indicated	RT-PCR, FC	↑
DU145 [94]	Human	100 U/mL	48 h	FC	↑
DU145 [112]	Human	10 ng/mL	24 h	WB, FC	↑
DU145 [119]	Human	100 U/mL	48 h	FC	↑
DU145 [152]	Human	10 ng	24 h	FC	↑
E006AA [94]	Human	100 U/mL	48 h	FC	↑
LASCPC [13]	Human	50 mg/mL	24 h	WB	↑
LAPC-4 [94]	Human	100 U/mL	48 h	FC	=
LNCaP [13]	Human	50 mg/mL	24 h	WB	=
LNCaP [94]	Human	100 U/mL	48 h	FC	=
LNCaP [128]	Human	10–100 ng/mL	24 h	FC	=
MyC-CaP [106]	Mouse	0.1–1–10 ng/mL	72 h	FC	↑
NCI-H660 [13]	Human	50 mg/mL	24 h	WB	↑
PC3 [10]	Human	Not reported	Not indicated	RT-PCR, FC	↑
PC3 [13]	Human	50 mg/mL	24 h	WB	↑
PC3 [94]	Human	100 U/mL	48 h	FC	↑
PC3 [103]	Human	20 ng/mL	24 h	WB, FC	↑
PC3 [112]	Human	10 ng/mL	24 h	WB, FC	↑
PC3 [119]	Human	100 U/mL	48 h	FC	↑
PC3 [128]	Human	10–100 ng/mL	24 h	FC	↑
PC3 [139]	Human	100 ng/mL	24 h	RT-PCR, WB	↑
PC3 [152]	Human	10 ng	24 h	FC	↑
TRAMP-C1 [106]	Mouse	0.1–1–10 ng/mL	72 h	FC	↑
TRAMP-C2 [126]	Mouse	0.5–10–20 ng/mL	48 h	FC	↑
TRAMP-C2 Ras [112]	Mouse	10 ng/mL	24 h	WB, FC	↑
Vcap [94]	Human	100 U/mL	48 h	FC	↑

↑ Upregulation/increase; = No alteration; FC: flow cytometry; RT-PCR: Real-Time Polymerase Chain Reaction analysis; WB: Western blot analysis.