FIG. 2.

Detailed presentation of the recombinant HBV reference plasmid panel. ID, clone identification number. Codon numbering is according to genotype A numbering. Three periods indicate a gap introduced between codons 529 and 548; sequences in this gap are not relevant for the design of the assay. The figure also shows the amino acid alignments in both the HBpol and HBsAg ORFs. Amino acids are indicated with the single-letter nomenclature; X, translational stop. The selected LiPA probes were based on this panel of clones; the positions of probes are not indicated. The strips with reactivity correspond to the strips shown in Fig. 4. Nb., number.