Figure 7.

Average number of L-929 cells per image section after culture for 24 h (left), 96 h (middle) and 168 h (right) on TCPS (dark grey) and FDM-printed PS substrates (light grey: smooth surfaces, white: rough surfaces). The cell number was assessed by the counting of Hoechst-stained cells. For the cultivation periods of 24 and 96 h, 1 × 10⁴ cells per well and for the cultivation period of 168 h, 2 × 10³ cells per well were seeded. Three independent experiments were carried out in triplicate. Four images per sample were taken (36 per condition per time point). Error bars represent the standard deviation.