Table 2.
Concentrations [µM] of genistein and its metabolites measured by LC-DAD in fermentation samples inoculated with pure cultures of Eggerthellaceae and Coriobacteriaceae strains. The initial concentration of genistein was 78.4 µM. Results are the mean values ± standard deviations of triplicates, except results labeled with § which are in duplicate. For their calculation, values between limit of detection (LOD) and limit of quantitation (LOQ) were set as LOD, and values <LOD were set as zero. DH-Genistein, Dihydrogenistein; 6′OH-O-DMA, 6′-Hydroxy-O-desmethylangolensin; 5-OH-Equol, 5-Hydroxy-equol; SD, standard deviation; -, not detected or values below LOD.
| T (h) | Genistein | DH-Genistein | 5-OH-Equol | 6′OH-O-DMA | |
|---|---|---|---|---|---|
| Control (medium without bacteria) # | 0 | 81.17 ± 0.80 | - | - | - |
| 24 | 79.05 ± 1.44 | - | - | - | |
| 48 | 74.91 ± 4.86 | - | - | - | |
| 72 | 75.78 ± 3.52 | - | - | - | |
| Adlercreutzia equolifaciens subsp. celatus DSM 18785T | 0 | 78.21 ± 9.11 | - | - | - |
| 24 | 4.32 ± 5.06 | 59.40 ± 0.81 | 2.05 ± 1.65 | - | |
| 48 | 14.67 ± 1.85 | 48.38 ± 3.19 | 1.78 ± 1.43 | - | |
| 72 | 42.33 ± 6.17 | 25.88 ± 6.96 | 1.49 ± 1.15 | - | |
| Adlercreutzia equolifaciens subsp. equolifaciens DSM 19450T | 0 | 78.17 ± 6.66 | - | - | - |
| 24 | 29.04 ± 26.03 | 41.61 ± 19.58 | - | - | |
| 48 | 2.15 ± 0.92 | 59.16 ± 1.17 | 1.42 ± 1.10 | - | |
| 72 | 14.99 ± 5.16 | 45.90 ± 4.00 | 1.83 ± 0.22 | - | |
| Adlercreutzia mucosicola DSM 19490T | 0 | 79.21 ± 1.15 § | - § | - § | - § |
| 24 | 0.75 ± 0.99 § | 48.76 ± 7.63 § | 10.02 ± 3.54 § | - § | |
| 48 | 0.72 ± 0.95 § | 26.77 ± 10.20 § | 20.17 ± 4.81 § | - § | |
| 72 | 7.22 ± 0.58 § | 16.89 ± 9.19 § | 20.33 ± 4.20 § | - § | |
| ‘Hugonella massiliensis’ DSM 101782T | 0 | 84.05 ± 2.44 | - | - | - |
| 24 | 1.94 ± 3.27 | 16.99 ± 14.19 | 27.36 ± 10.17 | - | |
| 48 | in one sample * | 0.91 ± 1.48 | 33.93 ± 2.40 | - | |
| 72 | in one sample * | in three samples * | 32.28 ± 2.80 | - | |
| Senegalimassilia faecalis KGMB 04484T | 0 | 90.96 ± 4.59 | - | - | - |
| 24 | 67.93 ± 8.13 | - | - | - | |
| 48 | 71.16 ± 0.37 | - | - | - | |
| 72 | 71.57 ± 1.36 | in three samples * | - | - | |
| Slackia equolifaciens DSM 24851T # | 0 | 70.47 ± 7.56 | - | - | - |
| 24 | 42.99 ± 2.42 | 29.83 ± 1.63 | - | - | |
| 48 | 25.58 ± 0.99 | 42.12 ± 1.65 | - | - | |
| 72 | 34.66 ± 0.22 | 33.97 ± 0.33 | - | - | |
| Slackia exigua DSM 15923T | 0 | 73.80 ± 3.44 | - | - | - |
| 24 | 31.44 ± 10.95 | - | - | 12.74 ± 1.23 | |
| 48 | 10.14 ± 12.55 § | - § | - § | 5.08 ± 0.68 § | |
| 72 | 20.52 ± 16.91 § | - § | - § | - § | |
| Slackia isoflavoniconvertens DSM 22006T | 0 | 75.31 ± 6.70 § | - § | - § | - § |
| 24 | 36.60 ± 12.03 | 39.13 ± 9.73 | - | - | |
| 48 | 7.17 ± 2.25 | 59.27 ± 1.68 | - | - | |
| 72 | 9.13 ± 0.41 | 55.71 ± 0.11 | - | - |
* Detected values ≥ LOD, but below LOQ. # Reported values are from one representative experiment (n = 3).