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. 2021 Nov 6;13(11):787. doi: 10.3390/toxins13110787

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative levels of GSH in proximal tubule epithelial cells. GSH levels were determined by the reaction with DTBN to form 5′-thio-2-nitrobenzoic acid in cells homogenates. (A) LLC-PK1 cells H₂O₂ (75 μM), TBHP (28 μM), and OTA (1 μM); (B) HK-2 H₂O₂ (2000 μM), TBHP (375 μM), and OTA (0.125 μM). Values are mean (n = 3) ± standard deviation, and they are expressed as percentage of control. Different letters indicate a statistically significant difference (p < 0.05) between treatments at each time by Tukey’s multiple comparison test.