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. 2021 Nov 17;22(22):12400. doi: 10.3390/ijms222212400

Figure 4.

Figure 4

Testing of interactions between the C2H2 proteins and the mutant variants of the CP190 protein in a yeast two-hybrid assay. (a) Schematic representation of Pita and Su(Hw) domain structures with CP190 BTB domain-interacting regions depicted. (b) Interaction of the C2H2 proteins with CP190 BTB domains bearing single amino acid substitutions within the peptide-binding groove. (c) Interaction of the C2H2 proteins with full-length CP190 proteins bearing single amino acid substitutions within the BTB domain. CP190 BTB domain and full-length proteins were fused to the GAL4 DNA-binding domain (GAL4 BD); C2H2 proteins were fused with the GAL4 activation domain. The growth assay yeast plates are shown in the Supplementary Materials Figures S10 and S11.