FIG. 1.

Expression of α1³¹² in hepatocytes. (A) Primary rat hepatocytes in culture were infected with Ad.α1³¹² or Ad.null at either 3 or 30 PFU/cell. Cell lysates from hepatocytes harvested 18 or 24 h postinfection were analyzed by Western blotting for expression of the recombinant α1³¹² protein (using an anti-Myc antibody) or the endogenous AMPK subunits (using either an anti-α antibody which recognizes both α1 and α2 isoforms, but not the truncated α1³¹² protein, or an anti-β or anti-γ antibody). (B) Cell lysates from hepatocytes infected with Ad.α1³¹² (30 PFU/cell for 24 h) were immunoprecipitated (IP) with either an anti-γ antibody or an anti-Myc antibody. Immune complexes were resolved by SDS-PAGE, and the presence of the β and γ subunits was determined by Western blotting. In each case, a representative blot from two independent experiments is shown.