FIG. 3.

Expression of α1³¹² inhibits the transcription of glucose-activated genes in cultured hepatocytes. Hepatocytes were grown in medium containing either 5 mM glucose and 100 nM insulin (G5) or 25 mM glucose and 100 nM insulin (G25) and were infected with Ad.α1³¹² (3 or 30 PFU/cell) or Ad.null (30 PFU/cell) and incubated for 18 h. At the end of this period, hepatocytes were treated with or without AICA riboside (AICAR) (250 μM) and incubated for 4 h. Total RNA was extracted, electrophoresed on a 1% agarose gel, and subjected to Northern blot analysis using cDNA probes encoding either FAS, L-PK, ACC, S14, albumin, or GAPDH. Blots were washed extensively and exposed to autoradiographic film at −70°C for 1 to 2 days. The blot shown is representative of two independent experiments.