FIG. 7.

Effect of extracellular glucose concentration on AMPK activity in hepatocytes. Hepatocytes were cultured overnight in medium containing 5 mM glucose and 100 nM insulin (G5). Medium was removed, and fresh G5 medium or medium containing 25 mM glucose and 100 nM insulin (G25) was added. Hepatocytes were harvested following incubation for a further 1 or 6 h. AMPK activity present in immune complexes isolated using either an anti-α1 (shaded bars) or anti-α2 (hatched bars) antibody was determined by phosphorylation of the SAMS peptide. Activities are plotted as a percentage of AMPK activity present in hepatocytes maintained in G5 and are the mean ± the SEM of three experiments. The 100% value for the activity in G5 is indicated by the dashed line.