Table 6.

Advantages and disadvantages of spheroid culture.

Advantage [371,372,373,376,377,378,379,380,381]	Disadvantage [359,366,376,377,378,379,380,381]
Inexpensive High efficiency Improves cell viability and proliferation Retains intrinsic phenotypic property Keeps physical interactions that more closely reflect behavior in the three-dimensional native tissue (3D)	Variable diameter and size Intense work Diffusion gradient depends on the size (oxygen nutrient, paracrine factor) that decreases inwards Self-disassembly is affected by the rate of production and consumption of factors