Figure 1.
Assembly of the DLR (Dual Luciferase Reporter) system adapted for A. gossypii: (A) Integrative cassette for the expression of the Renilla luciferase under the control of the PGPD1 promoter; (B) Integrative cassette for the expression of the firefly luciferase under the control of the different experimental promoters. All the modules were assembled into a destination vector following a Golden Gate method. The integrative cassettes were obtained by SapI digestion and used for A. gossypii transformation. Genomic integration occurred by homologous recombination.