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Confirmation of CRISPR construct through restriction digestion. The digested (ddH2O 15 μL + buffer 2 μL + plasmid 2 μL + HindIII 1 μL + 4 μL dye = 24 μL run) and undigested (1 μL plasmid + 4 μL ddH2O + 1 μL dye = 6 μL) reactions were loaded in 1% agarose gel. The digested band size is ~2000 bp. M is 1 kb DNA ladder (Thermo Fisher Scientific, Waltham, MA, USA).
