Figure 9.
In vitro evaluation of 10% GelMA-DDS efficacy against broth culture of S. aureus. A 0.5 McFarland of S. aureus suspension in MH broth was prepared and diluted 100 times to reach 5 × 106 CFU/mL. The broth suspension was added to each well to start the assay (0.5 mL per well), and a new broth solution was prepared every day, at every timepoint except for the last one, to refresh the bacteria solution. Optical density was measured at 600 nm each day for all groups: 3 µg, 15 µg, 30 µg, and 90 µg cefazolin in 10% GelMA. Control groups were added: 10% GelMA = negative control; S. aureus = negative control; positive control disc containing cefazolin 30 µg. Data are shown as mean ± SD. The experiment was performed three times with n = 6.