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Silver-stained SDS-PAGE loaded with 20 µg ES antigen obtained after worm cultivation in glucose containing RPMI medium, 0.9% NaCl or Ussing buffer and 2 µg CSO antigen per lane. Abbreviations: M, marker (PageRuler™ Plus Prestained Protein Ladder; Thermo Fisher Scientific, Schwerte, Germany); ES, excretory-secretory antigen; CSO, cuticular somatic antigen; H2O, bidistilled water.
