Table 1.
Glucose, amino acid and ammonia content of lyophilized native RPMI medium and lyophilized RPMI medium after in vitro cultivation of adult A. suum with untreated (containing total ES antigen products) and sealed mouth and anal openings (containing trans-cuticular antigen products only).
| Analyte | Native Lyophilized RPMI (mmol/L) | After Cultivation of Untreated Worms (mmol/L) | % Difference | After Cultivation of Sealed Worms (mmol/L) | % Difference |
|---|---|---|---|---|---|
| Glucose | 325.82 | <0.01 | >99.9 | 28.66 | 91.2 |
| Alanine | <0.01 | 1.22 | >99.2 | 3.17 | >99.7 |
| γ-aminobutyric acid (GABA) | <0.01 | 0.18 | >94.4 | 1.42 | >99.3 |
| Arginine | 20.44 | 6.22 | 69.6 | 16.26 | 20.5 |
| Asparagine | 5.68 | <0.01 | >99.8 | 0.06 | 98.9 |
| Aspartic acid | 2.23 | <0.01 | >99.6 | 0.56 | 74.9 |
| Glutamic acid | 1.81 | 1.62 | 10.5 | 7.77 | 76.7 |
| Glutamine | 29.26 | 4.94 | 83.1 | 14.14 | 51.7 |
| Glycine | 1.92 | 0.32 | 83.3 | 0.79 | 58.9 |
| Histidine | 1.21 | 0.54 | 55.4 | 0.91 | 24.8 |
| Isoleucine | 5.44 | 2.42 | 55.5 | 4.10 | 24.6 |
| Leucine | 5.38 | 1.88 | 65.1 | 3.76 | 30.1 |
| Lysine | 2.96 | 0.15 | 94.9 | 0.93 | 68.6 |
| Methionine | 1.16 | 0.23 | 80.2 | 0.66 | 43.1 |
| Ornithine | <0.01 | 1.37 | >99.3 | 0.40 | >97.5 |
| Phenylalanine | 1.17 | 0.34 | 70.9 | 0.64 | 45.3 |
| Proline | 1.97 | 0.92 | 53.3 | 1.76 | 10.7 |
| Serine | 3.92 | <0.01 | >99.7 | 0.05 | 98.7 |
| Threonine | 2.31 | <0.01 | >99.6 | 0.39 | 83.1 |
| Tyrosine | 1.40 | 0.36 | 74.3 | 0.91 | 35.0 |
| Valine | 1.87 | 1.08 | 42.2 | 1.58 | 15.5 |
| Ammonia | 48.5 mg/L | 471.4 mg/L | 89.7 | 377.2 mg/L | 87.1 |
Analytes obviously secreted by the worms are shown in bold for easier differentiation.