Table 3.
Compound parameters for the analysis of TTXs by HILIC-MS/MS.
| Analytes | Q1 Mass (m/z) | Q3 Mass (m/z) | Transition Type | DP (V) | CE (eV) | CXP (V) | Ion Ratio (Quant/Qual) |
|---|---|---|---|---|---|---|---|
| TTX and 4-epiTTX |
320 | 302 | Quant | 86 | 35 | 48 | TTX = 1.9 4 epiTTX = 3.0 |
| 162 | Qual | 86 | 59 | 26 | |||
| 4,9-anhydroTTX | 302 | 162 | Quant | 121 | 47 | 26 | 1.3 |
| 256 | Qual | 121 | 37 | 44 | |||
| 11-deoxyTTX | 304 | 286 | Quant | 86 | 35 | 22 | 6.3 |
| 176 | Qual | 86 | 47 | 28 | |||
| norTTX | 290 | 272 | Qual | 121 | 35 | 22 | / |
| 162 | Qual | 121 | 35 | 22 | |||
| TrideoxyTTX | 272 | 254 | Qual | 86 | 47 | 28 | / |
| 162 | Qual | 86 | 47 | 28 | |||
| C9-based-TTX (265) | 265 | 179 | Qual | 86 | 59 | 26 | / |
| 162 | Qual | 86 | 59 | 26 | |||
| C9-based-TTX (308) | 308 | 180 | Qual | 86 | 59 | 26 | / |
| 162 | Qual | 86 | 59 | 26 |
DP: declustering potential; CE: Collision energy; CXP: Collision cell eXit Potential Quant: quantitative transition; Qual: qualitative transition.