Figure 2.

Comparison of leaf dry biomass, root suberin content, and root suberin-associated wax amounts in wild-type (Col-0) plants. (a) Leaf biomass, (b) root poly-aliphatic suberin at different time points under control, drought, and re-watering conditions, and (c) chloroform-extractable root wax amounts from 5-week-old tap root surfaces, 3 weeks drought stressed versus control. The drought treatment was at 5% (v/v) water level. In (a,b), plants were subjected to re-watering (20% water level) after two weeks of drought treatment (i.e., 3 weeks = 2 weeks drought + 1 week re-watering and 4 weeks = 2 weeks drought + 2 weeks re-watering). Data points shown are mean values in grams of leaf dry mass from 10 replicate plants ± SE (a), milligrams of total poly-aliphatic suberin per gram of delipidated dry residue from five replicate samples (a replicate consisted of roots from a pool of 4 plants) ± SE (b), and micrograms of total root wax per gram of fresh weight from 4–5 replicate samples (a replicate consisted of 10 tap roots) ± SE (c). Asterisks indicate p < 0.05 by Student’s t-test comparing the values of the control and treatment. 1-OH, primary fatty alcohol; FA = free fatty acid.