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. 2021 Nov 16;22(22):12353. doi: 10.3390/ijms222212353

Figure 2.

Figure 2

Leptin relieves adipose tissue fibrosis by inhibiting ITGA5. (A) Proteomics sequencing analysis (n = 4). (B) The relative mRNA expression levels of ob/ob mice adipose tissue fibrosis-related genes COL1, COL3, MMP9, and ITGA5 (n = 4). (C) The relative content of hydroxyproline in adipose tissue of ob/ob mice (n = 4). (D) The relative mRNA expression levels of adipose tissue fibrosis-related genes COL1, COL3, MMP9, and ITGA5 in HFD mice (n = 4). (E) The relative content of hydroxyproline in adipose tissue of HFD mice (n = 4). (F) Afterwards, it is treated with leptin, the relative expression levels of ITGA5 mRNA in mice adipose tissue (n = 4). (G) After interference and overexpression of ITGA5 in mice, the expression efficiency of ITGA5 in adipose tissue (n = 4). (H) After interference and overexpression of ITGA5 in mice, relative mRNA expression levels of adipose tissue fibrosis-related genes COL1, COL3, MMP9 (n = 4). (I,J) After interference and overexpression of ITGA5 in mice, the relative mRNA expression levels of adipose tissue fibrosis-related genes COL3, MMP9 (n = 4). (K) After interference and overexpression of ITGA5 in mice, the relative content of hydroxyproline in adipose tissue (n = 4). (L) After interference and overexpression of ITGA5 in mice, Masson staining of mouse adipose tissue, scale bar: 300 μm, scale bar: 200 μm (n = 4). Values are means ± SD, * p < 0.05, ** p < 0.01 compared with the control group.