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. 2021 Nov 16;22(22):12353. doi: 10.3390/ijms222212353

Figure 3.

Figure 3

Leptin relieves fibrosis in adipocytes by inhibiting ITGA5. The cells were treated with TGF-β to construct a cell fibrosis model. Afterwards, it was treated with leptin. (A) The relative mRNA expression levels of COL1, COL3, COL6, MMP9, after TGF-β treated cells (n = 4). (B) The relative mRNA expression levels of fibrosis-related genes COL1, COL3, MMP9, and ITGA5 in adipocytes treated with TGF-β and leptin (n = 4). (C) The content of hydroxyproline after treating for TGF-β and leptin in adipocytes (n = 4). (D) The relative ITGA5 mRNA expression level after treatment with ITGA5 overexpression vector and interference fragments in adipocytes (n = 4). The cells were treated with TGF-β to construct a cell fibrosis model. Then the adipocytes were, respectively, treated with ITGA5 overexpression vector and interference fragments. (E,F) The relative expression levels of COL1, COL3, MMP9 mRNA, and COL3, MMP9 protein were detected (n = 4). (G) Immunofluorescence staining of COL3, scale bar: 200 μm (n = 4). (H) The content of hydroxyproline (n = 4). (I) The relative expression levels of COL1, COL3, MMP9 mRNA. Values are means ± SD, * p < 0.05, ** p < 0.01 compared with the control group.