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. 2021 Nov 16;22(22):12353. doi: 10.3390/ijms222212353

Figure 5.

Figure 5

Leptin promotes browning of fat tissues and improves the inhibitory effect of TGF-β on browning of adipocyte. Eight-week-old C57BL/6J mice were injected with leptin for 7 d and exposed to a cold environment at 4 °C for 24 h. After the mice were sacrificed in the neck, the total RNA and protein of the inguinal adipose tissue were extracted. (A) The relative mRNA expression levels of leptin and its receptor (n = 4). (B,C) The relative mRNA and protein expression levels of adipose tissue browning-related genes PRDM16, PGC-1α, UCP1 (n = 4). (D) HE staining of adipose tissue section, scale bar: 200 μm (n = 4). We used TGF-β to build a cell fibrosis model, and, under the conditions, treated with leptin, and then treated with an adrenergic receptor agonist (CL316, 243) to induce browning of adipocytes. (E) The relative mRNA expression levels of leptin and its receptor (n = 4). (F,G) The relative mRNA and protein expression levels of adipocyte browning-related genes PRDM16, PGC-1α, UCP1 (n = 4). (H) Immunofluorescence staining of UCP1, scale bar: 200 μm (n = 4). Values are means ± SD, * p < 0.05, ** p < 0.01 compared with the control group.