Figure 4.

The 12A12mAb-mediated neutralization of the N-terminal tau cleavage in brains from ICV-STZ mice is associated with reduction in AD-linked, pathologically relevant protein hallmarks. (A–C) Hippocampal homogenates from animals of four experimental groups (STZ 0 plus vehicle; STZ 0 plus mAb; STZ 3 plus vehicle; STZ 3 plus mAb) were analyzed by Western blotting by developing with antibodies reported alongside the blot; (B–D) semi-quantitative densitometry of the intensity signals of bands was carried out following normalization with β-actin level used as loading control. Arrows on the right side indicate the molecular weight (kDa) of bands calculated from migration of standard proteins. Values are from at least three independent experiments and statistically significant differences were calculated by one-way ANOVA followed by Bonferroni’s post-hoc test for multiple comparison among more than two groups. p < 0.05 was accepted as statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.0005).