Figure 9.

Histopathological alterations in the hippocampus of ICV-STZ mice are mitigated following 12A12mAb-mediated neutralization of the NH₂htau. (A) Representative images of hippocampal slices showing hematoxylin and eosin staining under examination with a light electric microscope at 4×. Scale bar = 50 μm. (B) 40× magnification of CA3 subfield. Notice that STZ alters the laminar organization and significantly increases the number of degenerating neurons with apoptotic disintegration of the nucleus (black arrows with heads). Following 12A12mAb treatment, neuroprotection is evident as shown by the presence of well-preserved hippocampal cyto-architecture characterized by viable neurons with healthy, not-damaged nuclei (arrows). Scale bar = 10 μm. (C) Immunofluorescence analysis (n = 4) at 40× magnification showing the distribution/expression of the NH₂htau peptide (green channel) in hippocampal CA3 regions from animals of four experimental groups (STZ 0 plus vehicle; STZ 0 plus mAb; STZ 3 plus vehicle; STZ 3 plus mAb). Nuclei were counterstained with DAPI (blue channel). Notice that, unlike not-immunized mice, the laminar organization/integrity is well preserved in STZ-treated hippocampi following 12A12mAb treatment in correlation with a significant diminution in signal of the NH₂htau (white arrows). Scale bar = 10 µm.