Figure 5.

Intravitreally administered CAR-peptide binds to preretinal tufts and to the blood vessels deeper in the retina. The CAR peptide was administered intravitreously. Whole eye cross sections were stained with Isolectin B₄ to label the blood vessels (in red) and the FAM-conjugated CAR-peptide is visible in green (A,B). The CAR-peptide was seen in the blood vessels (arrows) in different retinal layers, while some of the retinal blood vessels remained negative for CAR homing (arrowheads). CAR-homing was also seen in the neural retina, especially in GCL and INL layers (asterisks). Retina cross sections were also stained with anti-FITC antibody and the immunohistochemical signal was imaged by light microscope (C). The staining revealed strong CAR peptide homing/binding to preretinal neovascular tufts, but also some blood vessels in the deeper retina (arrows (C). (N = 2) GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS/OS, inner and outer segments. Scale bars represent 50 µm (A–C).