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. 2021 Oct 23;9(11):1236. doi: 10.3390/vaccines9111236

Figure 4.

Figure 4

Determination of antigen specific IgA from salivary washes and sera IgG after oral sublingual immunization with chimeric C1q conjugated VLPs. ELISA plates were coated with purified HIV Pr55 Gag or HIV SF162 Env at 3 µg/mL. Salivary washes from mice at 4 weeks post immunization were diluted 1:4 to determine (A) HIV Pr55 Gag specific and (B) HIV Sf162 Env specific IgA. (C) Sera IgG endpoint titer to CD40L/HIV VLPs. ELISA plates were coated with purified CD40L/HIV VLPs at 3 µg/mL and subsequently incubated with diluted sera from the indicated groups. n = 5 mice for each group. Error bars represent the standard deviation. * indicates p < 0.05 by One-way ANOVA and Tukey Post Hoc Test compared to PBS. # indicates p < 0.05 by One-way ANOVA and Tukey Post Hoc Test compared to CD40L/HIV VLPs.